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In situ analysis of multispecies biofilm formation on customized titanium surfaces

Identifieur interne : 004740 ( Main/Exploration ); précédent : 004739; suivant : 004741

In situ analysis of multispecies biofilm formation on customized titanium surfaces

Auteurs : V. Fröjd [Suède] ; L. Chávez De Paz [Suède] ; M. Andersson [Suède] ; A. Wennerberg [Suède] ; J. R. Davies [Suède] ; G. Svens Ter [Suède]

Source :

Molecular Oral Microbiology [ 2041-1006 ] ; 2011-08.

RBID : ISTEX:D032BA84F2B2C0D3A46FA0A6DDFB03FBA3D173EB

Descripteurs français

Wicri :
- topic : Consortium, Titane.

English descriptors

Abstract

Many studies to identify surfaces that enhance the incorporation of dental implants into bone and soft‐tissue have been undertaken previously. However, to succeed in the clinical situation, an implant surface must not support development of microbial biofilms with a pathogenic potential. As a first step in investigating this, we used two‐species and three‐species biofilm models with 16S ribosomal RNA fluorescence in situ hybridization and confocal laser scanning microscopy to examine the effect of surface characteristics on biofilm formation by species that can colonize titanium implants in vivo: Streptococcus sanguinis, Actinomyces naeslundii and Lactobacillus salivarius. Surfaces blasted with Al2O3 (Sa = 1.0–2.0 μm) showed a seven‐fold higher bacterial adhesion after 2 h than turned surfaces (Sa = 0.18 μm) whereas porous surfaces, generated by anodic oxidation (Sa = 0.4 μm), showed four‐fold greater adhesion than turned surfaces. Hence, increased roughness promoted adhesion, most likely through protection of bacteria from shear forces. Chemical modification of the blasted and oxidized surfaces by incorporation of Ca2+ ions reduced adhesion compared with the corresponding non‐modified surfaces. After 14 h, biofilm growth occurred in the three‐species model but not in the two‐species consortium (containing S. sanguinis and A. naeslundii only). The biofilm biovolume on all surfaces was similar, suggesting that the influence of surface characteristics on adhesion was compensated for by biofilm development.

Url:

https://api.istex.fr/document/D032BA84F2B2C0D3A46FA0A6DDFB03FBA3D173EB/fulltext/pdf

DOI: 10.1111/j.2041-1014.2011.00610.x

Affiliations:

Suède

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Le document en format XML

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 analysis of multispecies biofilm formation on customized titanium surfaces</title>
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<series><title level="j" type="main">Molecular Oral Microbiology</title>
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<term>Actinomyces naeslundii</term>
<term>Adhesion</term>
<term>Albrektsson</term>
<term>Albrektsson wennerberg</term>
<term>Anodic</term>
<term>Anodic oxidation</term>
<term>Appl environ microbiol</term>
<term>Average height deviation</term>
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<term>Bacterial species</term>
<term>Biomed mater</term>
<term>Biovolume</term>
<term>Biovolumes</term>
<term>Bloxca</term>
<term>Bloxca surfaces</term>
<term>Calcium ions</term>
<term>Clin</term>
<term>Clin implant dent relat</term>
<term>Clsm</term>
<term>Clsm images</term>
<term>Confocal laser scanning microscopy</term>
<term>Consortium</term>
<term>Corresponding surfaces</term>
<term>Dental implants</term>
<term>Dental plaque</term>
<term>Different surfaces</term>
<term>Different titanium surfaces</term>
<term>Frojd</term>
<term>Hybridization</term>
<term>Implant</term>
<term>John wiley sons</term>
<term>Lactobacillus</term>
<term>Lactobacillus salivarius</term>
<term>Laser</term>
<term>Malmo university</term>
<term>Microbial</term>
<term>Microbiol</term>
<term>Microbiology</term>
<term>Naeslundii</term>
<term>Oligonucleotide probes</term>
<term>Oral maxillofac implants</term>
<term>Oral microbiology</term>
<term>Osseointegration</term>
<term>Oxca</term>
<term>Oxca surfaces</term>
<term>Oxidized</term>
<term>Rough surfaces</term>
<term>Roughness</term>
<term>Rrna</term>
<term>Rrna fish</term>
<term>Salivarius</term>
<term>Sanguinis</term>
<term>Shear forces</term>
<term>Smooth surfaces</term>
<term>Streptococcus</term>
<term>Streptococcus mutans</term>
<term>Streptococcus sanguinis</term>
<term>Surface area</term>
<term>Surface characteristics</term>
<term>Surface roughness</term>
<term>Surface topography</term>
<term>Titanium</term>
<term>Titanium surfaces</term>
<term>Total biovolume</term>
<term>Uorescence</term>
<term>Wennerberg</term>
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<term>Actinomyces naeslundii</term>
<term>Adhesion</term>
<term>Albrektsson</term>
<term>Albrektsson wennerberg</term>
<term>Anodic</term>
<term>Anodic oxidation</term>
<term>Appl environ microbiol</term>
<term>Average height deviation</term>
<term>Bacterial adhesion</term>
<term>Bacterial growth</term>
<term>Bacterial species</term>
<term>Biomed mater</term>
<term>Biovolume</term>
<term>Biovolumes</term>
<term>Bloxca</term>
<term>Bloxca surfaces</term>
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<term>Clin</term>
<term>Clin implant dent relat</term>
<term>Clsm</term>
<term>Clsm images</term>
<term>Confocal laser scanning microscopy</term>
<term>Consortium</term>
<term>Corresponding surfaces</term>
<term>Dental implants</term>
<term>Dental plaque</term>
<term>Different surfaces</term>
<term>Different titanium surfaces</term>
<term>Frojd</term>
<term>Hybridization</term>
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<term>John wiley sons</term>
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<term>Malmo university</term>
<term>Microbial</term>
<term>Microbiol</term>
<term>Microbiology</term>
<term>Naeslundii</term>
<term>Oligonucleotide probes</term>
<term>Oral maxillofac implants</term>
<term>Oral microbiology</term>
<term>Osseointegration</term>
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<term>Rough surfaces</term>
<term>Roughness</term>
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<term>Rrna fish</term>
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<term>Shear forces</term>
<term>Smooth surfaces</term>
<term>Streptococcus</term>
<term>Streptococcus mutans</term>
<term>Streptococcus sanguinis</term>
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<term>Surface characteristics</term>
<term>Surface roughness</term>
<term>Surface topography</term>
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<front><div type="abstract" xml:lang="en">Many studies to identify surfaces that enhance the incorporation of dental implants into bone and soft‐tissue have been undertaken previously. However, to succeed in the clinical situation, an implant surface must not support development of microbial biofilms with a pathogenic potential. As a first step in investigating this, we used two‐species and three‐species biofilm models with 16S ribosomal RNA fluorescence in situ hybridization and confocal laser scanning microscopy to examine the effect of surface characteristics on biofilm formation by species that can colonize titanium implants in vivo: Streptococcus sanguinis, Actinomyces naeslundii and Lactobacillus salivarius. Surfaces blasted with Al2O3 (Sa = 1.0–2.0 μm) showed a seven‐fold higher bacterial adhesion after 2 h than turned surfaces (Sa = 0.18 μm) whereas porous surfaces, generated by anodic oxidation (Sa = 0.4 μm), showed four‐fold greater adhesion than turned surfaces. Hence, increased roughness promoted adhesion, most likely through protection of bacteria from shear forces. Chemical modification of the blasted and oxidized surfaces by incorporation of Ca2+ ions reduced adhesion compared with the corresponding non‐modified surfaces. After 14 h, biofilm growth occurred in the three‐species model but not in the two‐species consortium (containing S. sanguinis and A. naeslundii only). The biofilm biovolume on all surfaces was similar, suggesting that the influence of surface characteristics on adhesion was compensated for by biofilm development.</div>
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In situ analysis of multispecies biofilm formation on customized titanium surfaces

In situ analysis of multispecies biofilm formation on customized titanium surfaces

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri